In WBC pipets, you dilute up to what mark?

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Multiple Choice

In WBC pipets, you dilute up to what mark?

WBC pipets are designed to prepare a fixed dilution so that leukocytes fall into a countable range in the hemocytometer. On a WBC pipet, you fill with blood and dilute with fluid up to the 11 mark, which corresponds to about a 1:11 dilution (roughly 0.1 mL blood with 1.0 mL diluent to reach 1.1 mL total). This dilution gives a practical concentration of white cells for accurate counting and straightforward calculation of cells per microliter. Using the 11 mark avoids a dilution that’s too dilute (like 101, which would markedly reduce cell numbers and make counting unreliable) or too concentrated (other marks that yield around 10 or 1.1), which would distort the count and complicate calculations.

WBC pipets are designed to prepare a fixed dilution so that leukocytes fall into a countable range in the hemocytometer. On a WBC pipet, you fill with blood and dilute with fluid up to the 11 mark, which corresponds to about a 1:11 dilution (roughly 0.1 mL blood with 1.0 mL diluent to reach 1.1 mL total). This dilution gives a practical concentration of white cells for accurate counting and straightforward calculation of cells per microliter.

Using the 11 mark avoids a dilution that’s too dilute (like 101, which would markedly reduce cell numbers and make counting unreliable) or too concentrated (other marks that yield around 10 or 1.1), which would distort the count and complicate calculations.

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