Effect of overcharging on the cell count?

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Effect of overcharging on the cell count?

Overloading the sample pushes cell density beyond the counting system’s accurate range. When cells are too crowded, they crowd or pass through the detector in a group, making it hard to resolve individual cells. The counting method then records fewer events per unit volume than are truly present, giving a falsely decreased cell count. Proper dilution brings density into the accurate range and avoids this underestimation.

Overloading the sample pushes cell density beyond the counting system’s accurate range. When cells are too crowded, they crowd or pass through the detector in a group, making it hard to resolve individual cells. The counting method then records fewer events per unit volume than are truly present, giving a falsely decreased cell count. Proper dilution brings density into the accurate range and avoids this underestimation.

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