Depth of improved Neubauer chamber?

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Depth of improved Neubauer chamber?

Depth sets the known volume for each counted area, enabling the conversion from a cell count to a concentration. The improved Neubauer chamber is precisely constructed with a depth of 0.1 mm, so a 1 mm by 1 mm square contains 0.1 cubic millimeters of fluid, i.e., 0.1 μL. Counting cells in that fixed volume and applying the appropriate dilution factor allows you to calculate cells per microliter (or per milliliter) reliably. If the depth were different, the volume per counted square would change, and the same count would correspond to a different concentration. Therefore, 0.1 mm is the correct depth.

Depth sets the known volume for each counted area, enabling the conversion from a cell count to a concentration. The improved Neubauer chamber is precisely constructed with a depth of 0.1 mm, so a 1 mm by 1 mm square contains 0.1 cubic millimeters of fluid, i.e., 0.1 μL. Counting cells in that fixed volume and applying the appropriate dilution factor allows you to calculate cells per microliter (or per milliliter) reliably. If the depth were different, the volume per counted square would change, and the same count would correspond to a different concentration. Therefore, 0.1 mm is the correct depth.

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